Jacek Blazewicz1,
Anna Cysewska-Sobusiak2, Agata Lerczak2,
Marta Kasprzak1,
Wojciech T. Markiewicz3
1Institute of Computing Science,
Poznañ University of Technology,
ul. Piotrowo 3a, 60-965 Poznañ, Poland,
e-mail: blazewicz@sol.put.poznan.pl
2Institute of Electronics and Telecommunications,
Poznañ University of Technology,
ul. Piotrowo 3a, 60-965 Poznañ, Poland,
e-mail: cysewska@et.put.poznan.pl
3Institute of Bioorganic Chemistry,
Polish Academy of Sciences,
ul. Noskowskiego 12, 61-704 Poznañ,
Poland, e-mail: markwt.@ibich.poznan.pl
Abstract:
In the paper the problem of DNA sequencing by hybridization (SBH) is considered. With the developed software, MELTEM, several assembling procedures are used to ease a collecting a subset of oligonucleotides that would melt under practically identical conditions in a hybridization experiment. Some clustering approaches implemented in the program are compared. The algorithms of MELTEM are presented as well as the selected examples of a melting temperature analysis.
Keywords: DNA sequencing, clustering, hybridization experiment.