Research

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Institute of Bioorganic Chemistry PAN
ul. Noskowskiego 12/14
61-704 Poznan
Poland

Tel.: +48-61-8528503, ext. 114
Fax: +48-61-8520532
Email: wojtekr at ibch.poznan.pl

Prof. dr hab. Wojciech Rypniewski

Head of the Structure-Function Relationship in Biological Molecules Group at the Center for Biocrystallographic Research at the Institute of Bioorganic Chemistry of the Polish Academy of Sciences in Poznan.

- B.Sc. (Hons) 1983: University of York, England.
- Ph.D. 1986: Cambridge University, England.
- Habilitation 2001: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poland.
- Professor of chemistry 2007: entitled by the President Lech Kaczynski.

Publications

[60] Crystallographic and X-ray absorption spectroscopic characterization of Helicobacter pylori UreE bound to Ni2+ and Zn2+ reveal a role for the disordered C-terminal arm in metal trafficking. (2012), K. Banaszak, V. Martin-Diaconescu, M. Bellucci, B. Zambelli, W. Rypniewski, M.J. Maroney, and S. Ciurli, Biochem. J. 441:1017-1026 (doi:10.1042/BJ20111659). [Pub Med]

[59] Binding of the plant hormone kinetin in the active site of mistletoe lectin I from Viscum album. (2012) P.H. Malecki, W. Rypniewski, M. Szymanski, J. Barciszewski, and A. Meyer, BBA - Proteins and Proteomics 1824:334-338 (doi:10.1016/j.bbapap.2011.10.013). [Pub Med]

[58] Crystal structures of CGG RNA repeats with implications for fragile X-associated tremor ataxia syndrome. (2011), A. Kiliszek, R. Kierzek, W. Krzyzosiak, and W. Rypniewski, Nucleic Acids Res. 39:7308-7315 (doi:10.1093/nar/gkr368). [Pub Med]

[57] The crystal structures of eukaryotic phosphofructokinases from baker's yeast and rabbit skeletal muscle. (2011), K. Banaszak, I. Mechin, G. Obmolova, M. Oldham, S.H. Chang, T. Ruiz, M. Radermacher, G. Kopperschlaeger, W. Rypniewski, J. Mol. Biol. 407:284-297 (doi:10.1016/j.jmb.2011.01.019). [Pub Med]

[56] Crystallographic analysis of a thermoactive nitrilase. (2011), J.E. Raczynska, C.E. Vorgias, G. Antranikian and W. Rypniewski, J. Struct. Biol. 173:294-302 (doi:10.1016/j.jsb.2010.11.017). [Pub Med]

[55] Atomic resolution structure of CAG RNA repeats: structural insights and implications for the trinucleotide repeat expansion diseases. (2010), A. Kiliszek, R. Kierzek, W. Krzyzosiak, and W. Rypniewski, Nucleic Acids Res., 38:8370-8376 (doi:10.1093/nar/gkq700). [Pub Med]

[54] The hydration and unusual hydrogen bonding in the crystal structure of an RNA duplex containing alternating CG base pairs. (2010), D. A. Adamiak, J. Milecki, R. W. Adamiak, and W. Rypniewski, New J. Chem., 34:903-909 (doi:10.1039/b9nj00601j).

[53] Crystal structures of substrate- and sulfate-bound mouse thymidylate synthase. (2009), A. Dowiercial, A. Jarmula, W. Rypniewski, M. Sokolowska, T. Fraczyk, J. Ciesla, and W. Rode, Pteridines, 20:163-167.

[52] Structural insights into CUG repeats containing the 'stretched U-U wobble': implications for myotonic dystrophy. (2009), A. Kiliszek, R. Kierzek, W. Krzyzosiak, and W. Rypniewski, Nucleic Acids Res., 37:4149-4156 (doi:10.1093/nar/gkp350). [Pub Med]

[51] Structure of mistletoe lectin I from Viscum album in complex with the phytohormone zeatin. (2008), A. Meyer, W. Rypniewski, M. Szymanski, W. Voelter, J. Barciszewski, and Ch. Betzel, Biochim. Biophys. Acta, 1784:1590-1595 (doi:10.1016/j.bbapap.2008.07.010). [Pub Med]

[50] Non-canonical G(syn)-G(anti) base pairs stabilized by sulphate anions in two X-ray structures of the (GUGGUCUGAUGAGGCC) RNA duplex. (2008), W. Rypniewski, D. A. Adamiak, J. Milecki, and R. W. Adamiak, RNA, 14:1845-1851 (doi:10.1261/rna.1164308). [Pub Med]

[49] High resolution crystal structure of Rubrivivax gelatinosus cytochrome c'. (2008), S. Benini, W. Rypniewski, K. S. Wilson, and S. Ciurli, J. Inorg. Biochem., 102:1322-1328 (doi:10.1016/j.jinorgbio.2008.01.017). [Pub Med]

[48] The mistletoe lectin I - phloretamide structure reveals a new function of plant lectins. (2007), A. Meyer, W. Rypniewski, L. Celewicz, V. A. Erdmann, W. Voelter, T. Singh, N. Genov, J. Barciszewski, and Ch. Betzel. Biochem. Biophys. Res. Commun., 364:195-200 (doi:10.1016/j.bbrc.2007.09.113). [Pub Med]

[47] The crystal and solution studies of glucosamine-6-phosphate synthase from Candida albicans. (2007), J. Raczynska, J. Olchowy, P. V. Konariev, D. I. Svergun, S. Milewski, and W. Rypniewski, J. Mol. Biol., 372:672-688 (doi:10.1016/j.jmb.2007.07.002). [Pub Med]

[46] A synchrotron redetermination of 2-(morpholinium-4-yl)ethanesulfonate monohydrate, including a disordered water molecule. (2007), M. Kubicki, D. A. Adamiak, W. R. Rypniewski, and A. Olejniczak, Acta Crystallogr. E, 63:2604-2606 (doi:10.1107/S1600536807018685).

[45] Comparative crystallization and preliminary X-ray diffraction studies of locked nucleid acid and RNA stems of a tenascin C-binding aptamer. (2006), Ch. Foerster, A. B. E. Brauer, S. Brode, K. S. Schmidt, M. Perbandt, A. Meyer, W. Rypniewski, J. Betzel, Ch. Kurreck, J. P. Fuerste, and V. A. Erdmann, Acta Crystallogr. F, 62:665-668 (doi:10.1107/S1744309106020343). [Pub Med]

[44] The first crystal structure of an RNA racemate. (2006), W. Rypniewski, M. Vallazza, M. Perbandt, S. Klussmann, L. J. DeLucas, Ch. Betzel, and V. A. Erdmann, Acta Crystallogr. D, 62:659-664 (doi:10.1107/S090744490601359X). [Pub Med]

[43] Crystallisation and preliminary X-ray analysis of the isomerase domain of glucosamine-6-phosphate synthase from Candida albicans. (2005), J. Olchowy, R. Jedrzejczak, S. Milewski, and W. Rypniewski, Acta Crystallogr. F, 61:994-996 (doi:10.1107/S174430910503318X). [Pub Med]

[42] X-ray crystal and ab initio structures of 3',5'-di-O-acetyl-N(4)-hydroxy-2'-deoxycytidine and its 5-fluoro analogue: models of the N(4)-OH-dCMP and N(4)-OH-FdCMP molecules interacting with thymidylate synthase. (2005), A. Jarmula, W. R. Rypniewski, K. Felczak, and W. Rode, Structural Chemistry, 16:541-549.

[41] Synthetic human prion protein octapeptide repeat binds to the proteinase K active site. (2004) D. N. Georgieva, W. R. Rypniewski, H. Echner, M. Perbandt, M. Koker, J. Clos, L. Redecke, R. Bredehorst, W. Voelter, N. Genov, and Ch. Betzel, Biochem. Biophys. Res. Commun., 325:1406-1411. [Pub Med]

[40] Structure of the reduced disulpfide-bond isomerase DsbC from Escherichia coli. (2004), K. Banaszak, I. Mechin, G. Frost, and W. R. Rypniewski, Acta Crystallogr. D, 60:1747-1752. [Pub Med]

[39] Asp49 phospholipase A2-elaidoylamide complex: a new mode of inhibition. (2004), D. N. Georgieva, W. R. Rypniewski, A. Gabdoulkhakov, N. Genov, and Ch. Betzel, Biochem. Biophys. Res. Commun., 319:1314-1321. [Pub Med]

[38] Molecular details of urease inhibition by boric acid: Insights into the catalytic mechanism. (2004), S. Benini, W. R. Rypniewski, K. S. Wilson, S. Mangani, and S. Ciurli, J. Am. Chem. Soc., 126:3714-3715. [Pub Med]

[37] The X-ray structure of a snake venom Gln48 phospholipase A2 at 1.9 A resolution reveals anion-binding sites. (2004), D. N. Georgieva, M. Perbandt, W. Rypniewski, K. Hristov, N. Genov, and Ch. Betzel, Biochem. Biophys. Res. Commun., 316:33-38. [Pub Med]

[36] First look at RNA in L-configuration. (2004), M. Vallazza, M. Perbandt, S. Klussmann, W. Rypniewski, H. M. Einspahr, V. A. Erdmann, and Ch. Betzel, Acta Crystallogr. D, 60:1-7. [Pub Med]

[35] Trypsin revisited: Crystallography at (sub)atomic resolution and quantum chemistry revealing details of catalysis. (2003), A. Schmidt, Ch. Jelsch, P. Oestergaard, W. R. Rypniewski, and V. S. Lamzin, J. Biol. Chem., 278:43357-43362. [Pub Med]

[34] The 10.8 A structure of Saccharomyces cerevisiae phosphofructokinase determined by cryo-electron microscopy: localization of the putative fructose 6-phosphate binding sites. (2003), T. Ruiz, I. Mechin, J. Baer, W. R. Rypniewski, G. Kopperschlaeger, and M. Radermacher. J. Struct. Biol., 143:124-134. [Pub Med]

[33] Crystallization and preliminary X-ray diffraction studies of a toxic phospholipase A2 from the venom of Vipera ammodytes meridionalis complexed to a synthetic inhibitor. (2003) D. N. Georgieva, W. R. Rypniewski, M. Perbandt, M. Jain, N. Genov, and Ch. Betzel, Biochim. Biophys. Acta, 1650:1-3. [Pub Med]

[32] The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity. (2003), M. Perbandt, E. W. Guthoehrlein, W. R. Rypniewski, K. Idakieva, S. Stoeva, W. Voelter, N. Genov, and Ch. Betzel, Biochemistry, 42:6341-6346. [Pub Med]

[31] High resolution X-ray structure of the hyperthermophilic DNA-binding protein HU from Thermatoga maritima and the determinants of its thermostability. (2003), E. Christodoulou, W. R. Rypniewski, and C. E. Vorgias, Extremophiles, 7:111-122. [Pub Med]

[30] Crystallisation under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9 A resolution. (2002), R. Krauspenhaar, W. Rypniewski, N. Kalkura, K. Moore, L. DeLucas, St. Stoeva, A. Mikhailov, W. Voelter, and Ch. Betzel, Acta Crystallogr. D, 58:1704-1707. [Pub Med]

[29] Structure-based rationalisation of urease inhibition by phosphate: Novel insigths into the enzyme mechanism. (2001), S. Benini, W. R. Rypniewski, K. S. Wilson, S. Ciurli, and S. Mangani, J. Biol. Inorg. Chem., 6:778-790. [Pub Med]

[28] Preliminary X-ray diffraction studies of the external functional unit RtH2-e from the Rapana thomasianahemocyanin. (2001), M. Perbandt, V. Chandra, K. R. Rajashankar, K. Idakieva, K. Parvanova, W. Rypniewski, S. Stoeva, W. Voelter, N. Genov, and Ch. Betzel, Acta Crystallogr. D, 57:1663-1665. [Pub Med]

[27] Crystallization, preliminary X-ray analysis and amino acid sequence studies of an "external" functional unit from the Rapana thomasiana grosse (mollusc, gastropod) hemocyanin. (2001), M. Perbandt, V. Chandra, K. Idakieva, K. Parvanova, W. Rypniewski, S. Stoeva, W. Voelter, N. Genov, and Ch. Betzel, J. Cryst. Growth, 232:353-360.

[26] 1.9 A X-ray structure of 2'-OMe(CGCGCG)2 duplex shows dehydrated RNA with 2-methyl-2,4-pentanediol in the minor groove. (2001), D. A. Adamiak, W. R. Rypniewski, J. Milecki, and R. W. Adamiak, Nucleic Acids Res., 29:4144-4153. [Pub Med]

[25] Fusarium oxysporumtrypsin at atomic resolution, at 100K and 283K. A study of ligand binding. (2001), W. R. Rypniewski, P. Oestergaard, M. Noerregaard-Madsen, M. Dauter, and K. S. Wilson, Acta Crystallogr. D, 57:8-19. [Pub Med]

[24] Crystals structure of oxidised Bacillus pasteurii cytochrome c-553 at 0.97 A resolution. (2000), S. Benini, A. Gonzalez, W. R. Rypniewski, K. S. Wilson, J. J. van Beeumen, and S. Ciurli, Biochemistry, 39:13115-13126. [Pub Med]

[23] The complex of Bacillus pasteurii urease with acetohydroxamate anion from X-ray data at 1.55 A resolution. (2000), S. Benini, W. R. Rypniewski, K. S. Wilson, S. Miletti, S. Ciurli, and S. Mangani, J. Biol. Inorg. Chem., 5:110-118. [Pub Med]

[22] Structural properties of the nickel ions in urease: Novel insights into the catalytic and inhibition mechanisms. (1999), S. Ciurli, S. Benini, W. R. Rypniewski, K. S. Wilson, S. Miletti, and S. Mangani, Coord. Chem. Rev., 190:331-355.

[21] The crystal structure of the monomeric human SOD mutant F50E/G51E/E133Q at atomic resolution. The enzyme mechanism revisited. (1999), M. Ferraroni, W. R. Rypniewski, K. S. Wilson, M. S. Viezzoli, L. Banci, I. Bertini, and S. Mangani, J. Mol. Biol., 288:413-426. [Pub Med]

[20] A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels. (1999), S. Benini, W. R. Rypniewski, K. S. Wilson, S. Miletti, S. Ciurli, and S. Mangani, Structure, 7:205-216. [Pub Med]

[19] High resolution native and complex structure of thermostable beta-mannanase from Thermomonospora fusca - substrate specificity in glycosyl hydrolase family 5. (1998), M. Hilge, S. M. Gloor, W. Rypniewski, O. Sauer, T. D. Heightman, W. Zimmermann, K. Winterhalter, and K. Piontek, Structure, 6:1433-1444. [Pub Med]

[18] Crystallographic determination of reduced bovine superoxide dismutase at pH 5.0 and of anion binding to its active site. (1998), M. Ferraroni, W. R. Rypniewski, B. Bruni, P. Orioli, and S. Mangani, J. Biol. Inorg. Chem., 3:411-422.

[17] The complex of Bacillus pasteurii urease with beta-mercaptoethanol from X-ray data at 1.65 A resolution. (1998), S. Benini, W. R. Rypniewski, K. S. Wilson, S. Ciurli, and S. Mangani, J. Biol. Inorg. Chem., 3:268-273.

[16] Crystallization and preliminary high resolution X-ray diffraction analysis of native and beta-mercaptoethanol-inhibited urease from Bacillus pasteurii. (1998), S. Benini, S. Ciurli, W. R. Rypniewski, K. S. Wilson, and S. Mangani, Acta Crystallogr., D54:409-412. [Pub Med]

[15] Studies on synthesis and structure of O-b-D-ribofuranosyl(1''-2')ribonucleosides and oligonucleotides. (1998), W. T. Markiewicz, A. Niewczyk, Z. Gdaniec, D. A. Adamiak, Z. Dauter, W. Rypniewski, and M. Chmielewski, Nucleosides and Nucleotides, 17(1-3):411-424. [Pub Med]

[14] Crystallizaton and preliminary X-ray diffration analysis of cytochrome c' from Rubrivivax gelatinosus at 1.3 A resolution. (1998), S. Benini, W. R. Rypniewski, K. S. Wilson, and S. Ciurli, Acta Crystallogr., D54:284-287. [Pub Med]

[13] Crystallisation and preliminary X-ray analysis of the 12S form of phosphofructokinase from Saccharomyces cerevisiae. (1998), G. Obmolova, G. Kopperschlaeger, J. Heinisch, and W. R. Rypniewski, Acta Crystallogr., D54:96-98. [Pub Med]

[12] Crystal structure of the 2'-OMe(CGCGCG)2, an RNA duplex at 1.3 A resolution. Hydration pattern of the 2'-O-methylated RNA. (1997), D. A. Adamiak, J. Milecki, M. Popenda, R. W. Adamiak, Z. Dauter, and W. R. Rypniewski, Nucleic Acids Res., 25(22):4599-4607. [Pub Med]

[11] Crystals of cytochrome c-553 from Bacillus pasteurii show diffraction to 0.97 A resolution. (1997), S. Benini, S. Ciurli, W. R. Rypniewski, and K. S. Wilson, Proteins: Structure, Function and Genetics, 28:580-585. [Pub Med]

[10] Crystal structure of reduced bovine erythrocyte superoxide dismutase at 1.9 A resolution. (1995), W. R. Rypniewski, S. Mangani, B. Bruni, P. L. Orioli, M. Casati, and K. S. Wilson, J. Mol. Biol., 251:282-296. [Pub Med]

[9] Structure of inhibited trypsin from Fusarium oxysporum at 1.55 A. (1995), W. R. Rypniewski, C. Dambmann, C. von der Osten, M. Dauter, and K. S. Wilson. Acta Crystallogr., D51:73-84. [Pub Med]

[8] X-ray, NMR and molecular dynamics studies on reduced bovine superoxide dismutase: implications for the mechanism. (1994), L. Banci, I. Bertini, B. Bruni, P. Carloni, C. Luchinat, S. Mangani, P. L. Orioli, M. Piccioli, W. R. Rypniewski, and K. S. Wilson, Biochem. Biophys. Res. Commun., 202:1088-1095. [Pub Med]

[7] Evolutionary divergence and conservation of trypsin. (1994), W. R. Rypniewski, A. Perrakis, C. E. Vorgias, and K. S. Wilson, Protein Engng., 7:57-64. [Pub Med]

[6] Structural consequences of reductive methylation of lysine residues in hen egg while lysozyme: an X-ray analysis at 1.8 A resolution. (1993), W. R. Rypniewski, H. M. Holden, and I. Rayment, Biochemistry, 32:9851-9858. [Pub Med]

[5] Three-dimensional structure of myosin subfragment-1: a molecular motor. (1993), I. Rayment, W. R. Rypniewski, K. Schmidt-Baese, R. Smith, D. R. Tomchick, M. M. Benning, D. W. Winklemann, G. Wesenberg, and H. M. Holden, Science, 261:50-58. [Pub Med]

[4] The sequence and X-ray structure of the trypsin from Fusarium oxysporum. (1993), W. R. Rypniewski, S. Hastrup, Ch. Betzel, M. Dauter, Z. Dauter, G. Papendorf, S. Branner, and K. S. Wilson, Protein Engng., 6:341-348. [Pub Med]

[3] Crystallization and structure determination to 2.5 A resolution of the oxidized (2Fe-2S) ferredoxin isolated from Anabaena 7120. (1991), W. R. Rypniewski, D. R. Breiter, M. M. Benning, G. Wesenberg, Byung-Ha Oh, J. L. Markley, I. Rayment, and H. M. Holden, Biochemistry, 30:4126-4131. [Pub Med]

[2] The structure of unliganded E. coli phosphofructokinase at 2.4 A resolution. (1989), W. R. Rypniewski and P. R. Evans. J. Mol. Biol., 207:805-821. [Pub Med]

[1] The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution. (1987), H. M. Holden, W. R. Rypniewski, J. H. Law, and I. Rayment. The EMBO Journal, 6:1565-1570. [Pub Med]